Figure 12. RNA interference assay for eIF4E and S6K.

HCC and PH5CH8 cells were transfected with siRNA for eIF4E A) or S6K B) at a concentration of 100 nM. As controls, cells were transfected in parallel with siRNA scramble or mock-transfected. At 72 hours post-transfection, cells were infected with VSV-wt using an MOI of 0.1 and viral titers were determined 8 hours post-infection. Results are the average of three independent experiments, and error bars indicate the standard deviation. Mock-infected cultures were used to control the efficiency of the mRNA silencing by Western blot analyses. Western blot analysis was performed for each experiment.