Figure 13. RNA interference assay for eIF2B epsilon (eIF2Bε).

A) Western blot analysis of mock-infected cultures was performed for each experiment to assess the efficiency of RNA silencing. B) HepG2, Huh-7 and PH5CH8 cells were transfected with siRNA for eIF2Bε at a concentration of 100 nM. As controls, cells were transfected in parallel with control siRNA or mock-transfected. At 72 hours post-transfection, cells were infected with VSV-wt using an MOI of 0.1 for 8 hours. Results are the average of three independent experiments and error bars indicate the standard deviation.