Table 1. Primers used for RT-PCR and sequence analysis.

Gene	Sequence origin	Primers	Ta (°C)	Amplicon size (bp)	Application
Mouse Nanog	NM_028016	F: 5′-AGATGCGGACTGTGTTCTC-3′R: 5′-TGCGTTCACCAGATAGCC-3′	58	281	PCR/sequence
Canine NANOG	XM_543828	F: 5′-CCGTCTCTCCTCTTCCTTC-3′R: 5′-CACTGTTGCTCTCCTTTGG-3′	54,3	348	PCR/sequence
Porcine NANOG	NM_001129971	F: 5′-CTCTCCTCTTCCTTCCTC-3′R: 5′-ATCACACTGTTGCTATTCC-3′	58	345	PCR/sequence
Human NANOG	NM_024865	F: 5′-CCTCCAGCAGATGCAAGAACTC-3′R: 5′-GTAAAGGCTGGGGTAGGTAGGTG-3′	58	172	PCR/sequence
eGFP	EU541500	F1: 5′-CTGGTCGAGCTGGACGGCGACG-3′R1: 5′-CACGAACTCCAGCAGGACCATG-3′	59	630	PCR/sequence

F =  forward primer, R =  reverse primer. Ta =  annealing temperature used in PCR reaction.