Table 2. Antibodies used for immuno applications.

Immunogen	Source	Concentration	Description	Application
AA1-50 mouse NANOG	A300-398A (Bethyl)	10 µg/ml	Rabbit polyclonal	IHC mouse
Full length mouse NANOG fusion protein	AB21603 (Abcam)	1–2 µg/ml	Rabbit polyclonal	IF Dog, human
Synthetic peptide mouse NANOG	AB5731 (Chemicon)	1 µg/ml	Rabbit polyclonal	IB, IF pig
Human NANOG	14-5768 (eBioscience)	1–2 µg/ml	Mouse monoclonal	IF human
GFP (B-2)	SC9996 (Santa Cruz)	1∶500	Mouse monoclonal	IHC TNG mouse
Rabbit IgG	31460 (Pierce)	0.16 µg/ml	Goat polyclonal peroxidase conjugated	IB

AA =  Amino Acids, IHC =  immunohistochemistry, IF =  immunofluorescence, IB = immunoblotting.