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. Author manuscript; available in PMC: 2011 May 5.
Published in final edited form as: Cell Metab. 2010 Apr 22;11(5):427–437. doi: 10.1016/j.cmet.2010.04.002

Figure 2. dSH2B is required for lipid and glucose homeostasis in Drosophila.

Figure 2

(A) Fat bodies were isolated from WT and D/D adult flies (3 days), and lipid droplets were stained with Nile red. (B) Total triglycerides (TAG) were measured in adult flies (3 days) and normalized to total protein levels (32 flies per group). (C) Survival curves of starved flies (3 days) (160 flies per group). (D) Total TAG levels in adult flies (3 days) starved for various times (24 flies per group). (E) Tissue extracts were prepared from actin-GAL4/+ (act-Con) or actin-GAL4/UAS-dSH2B transgenic (act-dSH2B) adult flies (3 days), and immunoblotted with anti-dSH2B or anti-α-tubulin antibodies. (F) Whole body TAG levels were measured in actin-GAL4/+ and actin-GAL4/UAS-dSH2B adult flies (3 days) and expressed as % of actin-GAL4/+ TAG levels (32 flies per group). (G) Survival curves of starved flies (3 days)(120 flies per group). (H) Total TAG levels in starved adult flies (3 days) (32 flies per group). (I) Hemolymph carbohydrates in adult flies under fed conditions (60 flies per group). (J) The whole body carbohydrate levels in adult flies under fed conditions (32 flies per group). (K) Total head RNA was prepared from fed adult flies and used to measure dilp mRNA abundance (normalized to RPL32 expression) by quantitative RT-PCR (120 flies per group). *p< 0.05.

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