Table 2.

MT-PCR-HRM pooling strategy to accommodate for variable genome size.

Organism	1C genome (Mb)	No 384 well plates	No super pools	No matrix pools	No single pools	Total required simplex MT-PCR-HRM	Total required triplex MT-PCR-HRM
Brachypodium distachyon	300	24	1	10	54*	64	22
Potato	850	66	3	30	54	87	29
Barley	5500	430	17	30	54	101	35
Wheat	16000	1250	50	30	54	134	45
Swine	2600	203	8	30	54	92	31
Cattle	3100	242	10	30	54	94	32

Estimated number of pools and MT-PCR-HRM reactions required to find a single marker (or 3 markers simultaneously) within the denoted genomes, assuming a 3× coverage and 100 Kb average insert size for the corresponding BAC libraries. Note: *1× coverage = 18 single pools (see Figure 1); 3× coverage = 54 single pools