Figure 1.

Schematic structure and cloning strategies of TGF-β6. TGF-β6 precursor contains a signal peptide (SP), an N-terminal peptide (prodomain) and a C-terminal mature peptide. Partial sea bream TGF-β6 cDNA sequence was initially obtained by using a pair of degenerate primers TGF1 and TGF2. The 3' end was obtained by 3'RACE using primers TGF7 and TGF8. Several genomic clones, containing exons 2-5 and part of exon 6, were obtained as described in detail in the Methods section. The 5' end was obtained by 5' RACE using primers TGF15 and TGF16. For clarity the MSTN gene and deduced protein have not been represented in their real proportions. Primers sequences are presented in Additional file 1.