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Northern blot analysis of fish RNA hybridized with mouse TGF-β3 cDNA. Poly(A+)RNA (2.5 μg) extracted from S. aurata at different developmental stages (early embryo, blastula/gastrula stage; late embryos, shortly before hatching; 7d, larvae collected 7 days after hatching) was hybridized to mouse TGF-β3 cDNA as described in Methods. Ethidium bromide staining shows 18S and 26S rRNA as a loading control.
