Table 3.

Comparison of TGF-β exon sizes (bp) within the coding regions of vertebrate TGF-β genes

Species	Isoform	Exon1	Exon2	Exon3	Exon4	Exon5	Exon6	Exon7
S. aurata	TGF-β6	361	164	133	111	181	154	156
S. aurata	TGF-β1	595	235	151	111	48	-	-
O. mykiss	TGF-β	340	270	78	151	151	111	45
D. rerio	TGF-β3	346	164	130	108	172	154	156
D. rerio	predictedTGF-β2	349	164	133	111	178	151	156
G. aculeatus	predictedTGF-β	355	164	136	111	181	154	156
T. rubripes	predictedTGF-β2	364	164	133	111	175	154	156
T. rubripes	predictedTGF-β	361	164	133	111	181	154	156
O. latipes	predictedTGF-β2	361	164	133	111	181	154	156
T. nigroviridis	predictedTGF-β	361	164	133	111	181	154	156
H. Sapiens	TGF-β1	355	161	118	78	148	154	156
M. musculus	TGF-β2	346	164	133	111	178	154	156
H. Sapiens	TGF-β3	352	164	130	108	172	154	156
G. gallus	TGF-β2	343	164	133	111	175	154	156
G. gallus	TGF-β3	352	164	130	108	172	154	156
X. laevis	TGF-β5	334	158	118	75	151	154	156

Accession Nos (or gene ID from Ensembl): S. aurata TGF-β6, FJ966093; S. aurata TGF-β1, AF510084; O. mykiss TGF-β, AJ007836; D. rerio TGF-β3, AY744922 to AY744929; D. rerio predictedTGF-β2, LOC559723; G. aculeatus, predictedTGF-β, ENSGACG00000016971; T. Rubripes predictedTGF-β2, ENSTRUG00000008295; T. rubripes predictedTGF-β, ENSTRUG00000013167; O. latipes predictedTGF-β2, ENSORLG00000002336; T. nigroviridis predictedTGF-β, GSTENG10000619001; H. sapiens TGF-β1, X05839/X05840/X05843/X05844/X05849/X05850; H. sapiens TGF-β3, X14149; M. musculus TGF-β2, NM_009367; G. gallus TGF-β2, X59071/X59080/X59081/X59082; G. gallus TGF-β3, X58127/X60091/X60090; X. laevis TGF-β5, AF009331/AF009332/AF009333/AF009334/AF009335.

The novel TGF β6 in the six fish species is shown in bold letters. Identical exon length of the novel fish TGF-β6 in the six fish species is also shown in bold letters.