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Genotyping PfMSP6 using nested PCR and agarose gel electrophoresis. This study utilizes a nested PCR protocol to amplify the region of PfMSP6 where most inter- and intra-allele genetic diversity has been shown to occur. The two major PfMSP6 allele types, K1-class and 3D7-class alleles, result in nested PCR products of significantly different sizes, and allele genotyping was scored using agarose gel electrophoresis, as shown.
