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. Author manuscript; available in PMC: 2010 Jun 8.
Published in final edited form as: J Immunol. 2009 Dec 15;183(12):7787–7798. doi: 10.4049/jimmunol.0902318

FIGURE 7.

FIGURE 7

GMSC treatment attenuates colonic inflammatory responses but induces Treg responses in DSS-induced experimental colitis in C57BL/6 mice. Colitis was induced by oral administration of 3% DSS in drinking water for 7 days. Two × 106 of GMSCs or BMSCs in 200 μl of PBS were i.p. injected into mice 1 day after initiation of DSS treatment. Mice without any treatment (naive mice) or mice that received 200 μl of PBS served as controls. At day 10, mice were sacrificed. A, Immunofluorescence staining and Western blot analysis of the infiltrated CD4+ T lymphocytes in inflamed colons. B–E, Immunofluorescence staining and ELISA of IFN-γ, IL-17, IL-6, and IL-10 in inflamed colons. F, Immunofluorescence staining and Western blot analysis of FoxP3 in inflamed colon tissues. Scale bars, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, no significant difference (means ± SD). The results are representative of at least three independent experiments.