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. Author manuscript; available in PMC: 2010 Jun 8.
Published in final edited form as: J Immunol. 2010 Feb 3;184(5):2321–2328. doi: 10.4049/jimmunol.0902023

FIGURE 2.

FIGURE 2

The effect of supernatants from different T cell subtypes on the expression of ICAM-1 and VCAM-1 in MSCs. A, MSCs were cultured with Suppan-T, SupCD4, SupCD8, SupTh1, SupTh2, or SupTh17 at a 1:1 dilution. ICAM-1/VCAM-1 expression in MSCs was measured by flow cytometry 24 h after treatment, and median fluorescence intensity (MFI) was obtained. Data shown are means ± SD of a representative of three experiments. B, To determine the effect of different T cell supernatants on MSC-mediated immunosuppression, after 8 h of treatment with the supernatants as in A, the respective T cell blasts were added at a 20:1 ratio to MSC cultures along with IL-2 (200 U/ml). Cell proliferation was assessed by [3H]-TdR incorporation after an additional 8 h. Values are means ± SD of five replicate wells from a representative of three experiments.