A. Equal numbers of MCs were incubated in media containing either vehicle alone (control) or hemin (2.5 and 5.0 μM) for 24 hours followed by preparation of Western blots and probing for p21. The upper panel shows the effect of HO-1 induction on mesnagial cell p21 expression. The lower panel shows mesangial cell actin content under the same conditions.
B. Equal numbers of MCs were incubated in media containing either vehicle (control), hemin (5 μM), Hb (50 μM) + hemin or hemin + zinc protoporphyrin (100 μM) for 24 hours followed by preparation of Western blots and probing for p21. The upper panel shows the effect of Hb and ZnPP on hemin-induced mesangial cell p21 expression. The lower panel shows mesangial cell actin content under the same conditions.