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Trypsin-mediated breakdown of hydroxy-methaemoglobin as shown by loss of Soret band intensity and by SDS-PAGE.
The gel was stained with coomassie blue. Haemoglobin was at 4µM and trypsin at 2µM. Incubation was carried out at 37°C in 0.1M Tris-HCl, 0.14M NaCl, pH 7.5.
