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Proteolytic release of haem from bovine hydroxy-methaemoglobin-agarose mediated by trypsin and interpain A
Suspensions of methaemoglobin-agarose beads (4 µM with respect to haemoglobin tetramer) were incubated at 37°C with trypsin or InpA (2 µM) in 0.1M Tris-HCl, 0.14M NaCl, pH 7.5. Beads were pelleted by centrifugation and the supernatant buffer assayed at intervals for released free haem using the pyridine haemochrome assay.
