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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Biochem J. 2009 Dec 14;425(1):257–264. doi: 10.1042/BJ20090343

Table 1. Digestion fragments identified as arising from proteolysis of alpha and beta oxyhaemoglobin chains during InpA-mediated oxidation.

Oxyhaemoglobin (4 µM) was incubated at 37°C with 2µM InpA and samples removed periodically, incubated for 30 min with E-64 (0.5mM) to inhibit protease activity, and subjected to MALDI-TOF mass spectrometry (see text for details).

fragment
size (Da)
and time of
appearance		 corresponding
predicted
fragment size
(Da)		 Chain
designation
and residue
number		 amino acid sequence
1126.75
  (5h)		 1126.56 β-96 to 104 K…96LHVDPENFR104…
1356.92
  (4h)		 1356.65 β-46 to 59 F…46GDLSNPGAVMGNPK59…
1417.92
  (2h)		 1417.72 α-111 to 123 A…111VHLPNDFTPAVHA123…
1902.36
  (8h)		 1902.94 α-8 to 26 K…8TNVKAAWSKVGGHAGEYGA26…
2227.66
  (4h)		 2227.08 β-41 to 61 R…41FFDSFGDLSNPGAVMGNPKVK61…
2227.10 α-41 to 60 K…41TYFPHFDLSHGSAQVKAHGK60…
2037.65
  (2h)		 2037.97 β-86 to 103 F…86AALSELHCDKLHVDPENF103…