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dacn is not required for apoptotic chromatin destruction. (A-I) Micrographs of hemocytes isolated from Drosophila third instar larvae and cultured for 2 hours (A-C). After apoptosis was induced using Smac mimetic (Smac) (Li et al., 2004), wild-type cells (D-F) and dacn1/27 cells (G-I) had condensed and fragmented chromatin, as visualized by Hoechst staining (B,E,H) and TUNEL labeling (C,F,I). Genotypes: (A-F) w1118; (G-I) dacn27 nGFP33 nGFP38 FRT40A/dacn1.
