Fig. 4.

Endocytosis is not impaired in dacn, but dwell time in endosomes is reduced. (A-C′) Live Drosophila eye discs with mitotic clones of dacn²⁷ (–/–) were labeled with an antibody to the extracellular domain of Dl. Clones were marked with a double dose of nuclear GFP (A,B,C, outlined). Immediately after labeling, Dl labeling was indistinguishable in apical sections of wild-type and dacn²⁷ cells (A′) and not detected in basal sections (A′). After a 20-minute chase, Dl antibody signal was reduced in subapical regions of dacn clones compared with wild-type cells (B). After a 60-minute chase, Dl antibody signal was indistinguishable in wild-type and dacn²⁷ cells (C′). (D-D′) Texas-Red dextran was endocytosed into eye discs containing dacn²⁷ clones for 5 minutes and then chased for 20 minutes. Dextran trafficking was unchanged in dacn²⁷ cells. Genotypes: yw eyFLP/+; dacn²⁷ nGFP³³ nGFP³⁸ FRT40A/FRT40A. Scale bar: 10 μm.