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Disrupted gene expression by dmrt1 knockdown. The expression of the genes otx, six3/6, c-actin and foxC were assayed by RT-PCR in single embryos at early tailbud stage, and in pools of four embryos at late gastrula stage. Embryos were injected at the one-cell stage with either a dmrt1-MO or a control-MO (cont-MO). All samples included a no-reverse transcriptase control, which showed no amplification product (data not shown).
