METH induces apoptosis in some striatal neurons of the striatum. Double-labeled epifluorescent micrographs of striatal tissue stained with Cy3-labeled antibodies against NeuN and TUNEL with FITC-conjugated dUTPs in control (a–c) and METH-treated (30 mg/kg, i.p., killed 24 h post-treatment) animals (d–i). Bottom panels (c, f, i) are overlays of both TUNEL and NeuN staining. Higher magnification of METH-treated animals indicates that NeuN-positive neurons overlap with TUNEL-positive cells (g–i). White arrows point to overlapping TUNEL and NeuN positive cells. Scale bar=100 μm (a–f), 20 μm (g–i).