Table 1.
Selected genes up-regulated or down-regulated by sustained EGR-1 expression as delineated from Affymetrix microarray analysis
| UniGene ID | Entrez gene | Gene symbol | Entrez definition | EGR-1 (16 h) |
Control virus (16 h) |
EGR-1 (24 h) |
Control virus (24 h) |
EGR-1 (48 h) |
Control virus (48 h) |
|---|---|---|---|---|---|---|---|---|---|
| Transcriptional repression/modulation | |||||||||
| Hs.159223 | 4665 | NAB2 |
NGFI-A -binding protein 2
(EGR-1-binding protein 2) |
4.5 | −0.3 | 4.1 | 0 | 3.8 | −0.4 |
| Hs.107474 | 4664 | NAB1 |
NGFI-A -binding protein 1
(EGR-1-binding protein 1) |
2.9 | 0.2 | 3,1 | 0.1 | 3.0 | 0.8 |
| Hs.25292 | 3726 | JUNB | Jun B proto-oncogene | 2.6 | −0.2 | 2.8 | −0.2 | 3.4 | 0.2 |
| Hs.2780 | 3727 | JUND | Jun D proto-oncogene | 1.9 | 0.4 | 1.7 | −0.1 | 1.2 | 0.7 |
| Antiangiogenesis | |||||||||
| Hs.24395 | 9547 | CXCL14 | Chemokine (C-X-C motif) ligand 14 | 3.8 | 0 | 8.0 | 0.2 | 11.9 | 0.2 |
| Hs.446641 | 7076 | TIMP-1 |
Tissue inhibitor of metalloproteinase-1
(erythroid-potentiating activity) |
3 | 0 | 3.5 | 0.2 | 3.3 | 0.4 |
| Hs.245188 | 7078 | TIMP-3 |
Tissue inhibitor of metalloproteinase-3
(Sorsby fundus dystrophy) |
1.3 | −0.3 | 2.8 | 0.3 | 4.7 | 1.4 |
| Hs.347713 | 2321 | FLT1 | fms-related tyrosine kinase 1 | 1.3 | 0.2 | 1.5 | 0.6 | 1.4 | 0.5 |
| Hs.12337 | 3791 | KDR | Kinase insert domain receptor | −0.7 | 0.2 | −0.7 | 0.4 | −2.6 | 0.8 |
| Hs.77274 | 5328 | PLAU |
Plasminogen activator, urokinase |
−2.1 | −0.6 | −1.3 | −0.7 | −0.8 | −0.9 |
| Cell cycle arrest | |||||||||
| Hs.106070 | 1028 | CDKN1C |
Cyclin-dependent kinase inhibitor 1C
(p57, Kip2) |
3.2 | −0.3 | 3.8 | 0.2 | 5.8 | 0.4 |
| Hs.110571 | 4616 | GADD45B |
Growth arrest– and DNA
damage–inducible, β |
0.1 | −1.9 | 1.3 | −1.9 | 4.1 | −3.3 |
| Hs.1103 | 7040 | TGF-B1 |
Transforming growth factor-β1
(Camurati-Engelmann disease) |
1.9 | 0 | 1.0 | 1.1 | 2.0 | −0.3 |
| Proapoptosis | |||||||||
| Hs.1183 | 1844 | DUSP2 |
Dual-specificity phosphatase 2 |
4.3 | 0 | 6 | 0 | 5.8 | −0.4 |
| Hs.112058 | 10572 | SIVA | CD27-binding (Siva) protein | 1.8 | 0.2 | 2.6 | −0.6 | 3.9 | −0.1 |
| Hs.293225 | 162989 | DEDD2 | Death effector domain containing 2 | 1.8 | 0.3 | 2.4 | 0.3 | 2.8 | 0.5 |
| Others | |||||||||
| Hs.110675 | 348 | APOE | Apolipoprotein E | 7.4 | −0.2 | 8.5 | 0 | 9.8 | −0.3 |
| Hs.208229 | 84634 | GPR54 | G protein–coupled receptor 54 | 7.9 | −0.9 | 8.1 | −0.8 | 8.4 | 0.2 |
NOTE: Total RNA was isolated from HUVEC following infection with either EGR-1-expressing adenoviruses or empty control viruses at 16, 24, and 48 hours. The RNA samples were subjected to microarray analysis using the Affymetrix U133 GeneChip set. Gene expression changes relative to noninfected control cultures for the EGR-1, and control viruses are displayed on a logarithmic scale (log 2). The full set of Affymetrix data is available in GEO database under ID GSE2299. A short list of the most highly up-regulated genes is available as Supplementary Table S2 from the authors on request.