Figure 7.
Stable expression of mutant and wild-type NOP10. (A) Endogenous NOP10 expression is reduced with siRNA treatment but modest plasmid-derived NOP10 is detected in cell lines containing either wild-type or mutant plasmid. NOP10 expression in stable cell lines is shown relative to time = 0 in the cell line with the empty vector (empty vector, dotted bar; wild-type plasmid, vertical lines; mutant plasmid, horizontal lines). (B) TERC levels are partially rescued in the cell line expressing wild-type NOP10 but not in the cell line expressing the mutant NOP10. TERC expression is shown relative to time = 0 for each cell line.