Table 1.

Accession number, identity, forward primer sequence, reverse primer sequence, annealing temperature (T_a), and number of cycles (C) for quantitative RT-QPCR assays

EST Putative identity	Forward primer Reverse primer	Ta	C	Efficiency
AB122066	F: GGAAGCTGCTGAGATGGGAA			1.98±0.004
Elongation factor 1α	R: TCCAACACCCAGGCGTATTT
AJ305315	F: AGGGCATTTCATGTCCGAAG	58	40	2.01±0.002
Heat shock protein 70	R: TGCTCAGACATCCAAGGAAGG
AJ565456	F: ACCACAAAAGCAGTTGCCGT	58	40	2.08±0.001
S-crystallin	R: TGAGGGATAAGGCGACCATC
AJ565627	F: GTACTTCAATGAGATGCCGTGG	59	40	1.84±0.153
Nucleoredoxin	R: AGGTCACGTTCACTGAAGGGA
AJ565582	F: CCTTCGGATCGTCTGGAGAA	59	40	1.98±0.004
Delta-9 desaturase	R: CCGAAGTGTAAAAGAGCCATCAG
AM237676	F: GCCAAACCTCGCCTACCTTC	58	40	2.00±0.004
Peroxinectin	R: GTGGAGTTGACGCGTGACATA
AM237729	F: GAGATCGTGGAGGACAAGAAAGA	58	45	1.97±0.005
No match	R: CTGCTCCGATCTCGTCAGC
AM237796	F: GCTGTGGAGTGGTTACCAGGA	59	40	2.01±0.004
Galectin	R: GAGAGTAACCCAGCTCCCCG
BQ426456	F: AGGCCATTGAAGCCACAAAT	58	40	2.00±0.005
Neuropeptide Y	R: TCCACAAAGGACTCTTGCCAT
BQ426550	F: TGACGCAATGGATTTTCTGC	58	40	1.86±0.005
Heat shock protein 27	R: GCCTGGTATCCAGGTCGGTAG
BQ426623	F: GCGCGACATTTTCGATTTTC	59	40	1.88±0.007
No match	R: CCATTGGTGATGACGTAACGC
BQ426658	F: AGAAGTGTGGAGAAATTTTGTCCAG	59	40	1.92±0.005
No match	R: TGGGATCAGATCCCATGCTC
BQ426884	F: ACGATGTCAGGGACAACTTTCTGT	58	45	1.96±0.002
No match	R: CGCAGAGGTAATCTTTTAAACGTCA
BQ426927	F: GACATGGTCGTCCAGATCGG	59	40	2.02±0.005
SOCS2	R: CCACCCTGATTGACTTAACGTGA
CX069163	F: TATGAAGTAGCAGCATGATGACTCC	58	40	2.02±0.004
Collagen	R: TCGTGGCTACCTAATGGCG
CX069133	F: GTTCCTGCCACGACCTGAGT	58	40	2.01±0.004
Cystatin B	R: GCTGGACACTTACACCCCAGTC

Mean efficiency (E_r) ± SEM was calculated by using the software LinReg PCR (Ramakers et al. 2003). Target concentration was normalized to that of the endogenous control ef1 (AB122066)