Figure 9.
Comparison of the Y567A mutant anti-8-oxoG:dCTP and syn-8-oxoG:dATP ternary crystal structures. A. Superimposed palm domains showing that 8-oxoG flips from anti to a syn conformation resulting in a shift of A567 and G568 into the minor groove of the primer-template by ~ 0.6 Å. The Y567A:anti-8-oxoG:dCTP backbone is shown in orange, and the Y567A:syn-8-oxoG:dATP backbone in yellow. B. Schematic illustration of the spatial interaction between G568 and the nascent base-pair as shown in (A).