. 2010 Apr 8;61(10):2589–2601. doi: 10.1093/jxb/erq089

Table 2.

Expression analyses of marker genes for AM symbiosis and hormone pathways by quantitative real time RT-PCR (qPCR) in roots colonized by G. mosseae (Gm) or G. intraradices (Gi)

ID	Annotation	Pathway/marker	Microarray		qPCR
			Gm	Gi	Gm	Gi
BI933750	1-Deoxy-D-xylulose 5-phosphate synthase 2 (DXS-2)	AM	5.00^a	9.92^a	8.59^a	20.08^a
U30465	Class II chitinase (Chi2;1)	AM	2.41^a	3.01^a	2.70^a	2.23^a
Y15846	Pathogenesis protein PR10-like	AM	1.93^a	2.72^a	4.82^a	5.81^a
AY885651	Phosphate transporter LePT4	AM	–	–	1141.69^a	2046.48^a
U09026	Lipoxygenase A (LOXA)	Oxylipins	6.59^a	2.78^a	7.46^a	3.38^a
AF454634	Allene oxide synthase 3 (AOS3)	Oxylipins	8.49^a	3.42^a	16.60^a	6.28^a
AF317515	Divinyl ether synthase (DES)	Oxylipins	2.29^a	1.96^a	3.28^a	2.94^a
U37840	Lipoxygenase D (LOXD)	JA	1.27	2.17^a	0.79	2.35^a
AJ271093	Allene oxide synthase 1 (AOS1)	JA	2.14^a	2.52^a	2.51^a	3.32^a
AF230371	Allene oxide synthase 2 (AOS2)	JA	0.97	0.89	0.98	0.84
AF384374	Allene oxide cyclase (AOC)	JA	1.34	1.29	1.32	1.41
AJ278332	12-Oxophytodienoate 3 reductase (OPR3)	JA	0.94	1.05	1.09	1.15
AF083253	Multicystatin (MC)	JA	9.27^a	1.64	8.35^a	0.76
K03291	Proteinase inhibitor II (PinII)	JA	5.92^a	0.94	7.39^a	1.06
CN384809	1-Aminocyclopropane-1-carboxylic acid oxidase (ACO1)	ET	2.35^a	2.27^a	0.79	1.76
X58885	Ethylene-forming enzyme (EFE)	ET	0.69	1.13	0.61	1.54
AY394002	CTR1-like protein kinase (CTR4)	ET	1.11	1.13	0.71	0.83
Z97215	9-cis-Epoxycarotenoid (NCED1)	ABA	0.96	0.69	0.68	0.57
X51904	Le4	ABA	–	–	0.82	0.48
M69247	Pathogenesis-related protein PR1a	SA	ND	ND	C_t 30.67	ND

Numbers indicate fold change in expression levels compared with non-mycorrhizal controls.

C_t, the threshold cycle; ND. non-detected;– not present in the tomato array.

Significant (P <0.01) changes.