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. 2010 Jun 8;5(6):e10977. doi: 10.1371/journal.pone.0010977

Figure 2. Intracellular localization of Pdro.

Figure 2

(A) Pdro-GFP (green) expressing SHEP cells were either incubated with Lysotracker for 30 min and fixed before mounting, or fixed, permeabilized, and incubated with antibodies to EEA-1, LBPA and Lamp-1 to label endosomal compartments. For LE/LY labelling with Dextran, Pdro-GFP expressing SHEP cells were incubated with Alexa Fluor 488 Dextran. Co-distribution was assessed by confocal microscopy and is visualized in yellow in the merged images. Boxed areas are shown magnified. Scale bar = 10 µm. (B) Immunofluorescence images show intracellular localization of Pdro in SHEP cell lines stably expressing Pdro-GFP, and ΔPdro-GFP using antibodies directed to GFP. Scale bar = 10 µm. Cellular lysates from these cell lines were subjected to SDS-PAGE and immunoblotted with antibodies against GFP tag (Right panel). Molecular size standards (in kD) are shown on the left of the panel.