. 2010 Jun 8;5(6):e10977. doi: 10.1371/journal.pone.0010977

Table 1. Pdro knockdown increases cellular cholesterol content.

	Scramble	SiPdro
Filipin intensity (arbitrary units)^a	432.33±1.61	811.12±61.49 ^*
FITC-WGA fluorescence (arbitrary units)^a	117.14±6.75	121.74±1.64
Free cholesterol (µg/mg protein)^b	3.25±0.93	6.27±1.48 ^*

a: Filipin and FITC-WGA labelling of the cells was quantified by flow cytometry (arbitrary units of fluorescence intensity).

b: Free cholesterol was quantified by gas chromatographic analysis after separation of cellular lipids by TLC. Values are mean ± SD of three independent experiments en triplicate wells.

*p≤0.05 using Mann Whitney test.