Figure 2. NOMPC localization in abdominal bristles from wild type or nompC mutants.

Abdominal cuticles from wild type (A) or nompC mutants (B–C) pharate adults were doubly stained with anti-NOMPC (magenta) and anti-EYS (mAb 21A6, green) antibodies. Focal anti-NOMPC signals at bristle bases (arrowheads in A) were seen in wild type, but not in nompC null (B). In nompC⁴ (C), reduced NOMPC foci are present in the bristle bases (arrow heads in C), distal to the EYS foci, as in wild type; but a significant amount of signal is also seen in the cell body region (outlined by dotted lines in C). Scattered non-specific signals are seen in both wild type and mutant cuticles. The arrows indicate anti-EYS staining at the basal end of the sensory cilia. Note: the gain of magenta channel in B and C is much higher than that in A. Scale bars represent 10 µm.