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. 2010 Jun 8;5(6):e11012. doi: 10.1371/journal.pone.0011012

Figure 5. NOMPC is mislocalized in btv mutant cilia.

Figure 5

Antennal chordotonal neurons of wild type (A) or btv mutant (B) were stained with anti-NOMPC antiserum (red). Cilia were labeled with a functional GFP-tagged NOMPB/IFT88 [24] (green). The neuronal cell bodies and inner segments were counter-stained with mAb 22C10 (blue). NOMPB/IFT88 is normally detected only in the proximal segment, whereas NOMPC is only in the distal segment of the sensory cilia (A). In btv, however, NOMPB/IFT88 and NOMPC distributions overlap along the whole cilia, especially accumulating at the base of cilia (arrow heads) (B). NOMPC signal is also seen in cell body regions (yellow bracket in B). Note: the ciliary accumulation of NOMPB/IFT88 is much higher in btv mutant than wild type. Scale bars represent 5 µm.