Adoptively transferred BMDCs were detected in the lymphoid organs and pancreatic tissue. BMDCs were pulsed with β cell Ag, labeled with CFSE, left uncoated or coated with control or anti-CTLA-4 Ab, and injected i.v. into pre-diabetic female NOD mice (5×106 cell/mouse). One group of mice that did not receive DCs (non-recipient group) were used as background controls. Mice were euthanized after 24 h, single cell suspension of spleen, pancreatic LN and pancreata were examined for CD11c+CFSE+ cells by FACS. Representative scatter plots (gated for CD11c+ population) of three mice/group tested individually are shown. Percentage of CFSE+ population among CD11c+ cells is shown on each scatter plot.