Skip to main content
. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: J Immunol. 2010 May 14;184(12):6695–6708. doi: 10.4049/jimmunol.0903130

FIGURE 5.

FIGURE 5

DC directed CTLA-4 engagement induces suppressor cytokine producing hypo-proliferative T cells against β-cell Ag. Eight week old euglycemic female NOD mice were left untreated or treated with antigen-pulsed control or anti-CTLA-4 Ab coated DCs as described for Fig. 3. Cells from untreated (control) and DC recipient mice were examined ex vivo for antigen specific T cell response 15 days after second injection with DCs. A). Spleen and PnLN cells were incubated with β-cell Ag and the spent media collected from 72 h cultures were tested for IFN-γ, IL-4, IL-17, IL-10, and TGF-β1 by multiplex assay or ELISA. B) CFSE labeled spleen and PnLN cells were incubated with β cell Ag for 5 days, CFSE dilution in CD4+ population was examined by FACS after staining using fluorochrome-labeled CD4 specific Ab. Mean±SD values of cells from at least 5 mice tested in triplicate are shown for panels A and B. Representative scatter plots are also shown for panel B.