Fig. 3.

Zn and Fe level of osteoblastic cell culture media components using TPEN and DTPA as Zn chelators (n=2)

Five µM TPEN and 1.2 mM DTPA were the optimized concentration used to chelate Zn. Different letter superscripts mean significantly different between media composition at p<0.05 by one-way ANOVA. [A, αMEM; B, growth media; C, osteogenic differentiation media + TPEN or DTPA^*; D, 0 µM ZnCl₂ + TPEN or DTPA; E, 3 µM ZnCl₂ + TPEN or DTPA; F, 6 µM ZnCl₂ + TPEN or DTPA; G, 9 µM ZnCl₂ + TPEN or DTPA; H, 12 µM ZnCl₂ + TPEN or DTPA; I, 15 µM ZnCl₂ + TPEN or DTPA].