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. Author manuscript; available in PMC: 2010 Jun 9.
Published in final edited form as: Mol Cancer Res. 2009 Jun 9;7(6):955–965. doi: 10.1158/1541-7786.MCR-08-0445

Figure 5. MycΔp85α expression inhibits xenografts tumor growth rate and Ki67 staining but has no effect on the level of PARP cleavage.

Figure 5

Parental HT29 cells and clone Δ15 cells were grown as tumor xenografts on Balb/c-nude mice and tumors were measured three times a week by callipers. Once tumors reached 300 mm3 mice were switched onto feed containing dox or control-feed. Four animals were sacrificed three days after being switched onto dox/control-feed and their tumors were bisected and either snap frozen or fixed in 10% formalin. A further six mice from each group were used for tumor growth analysis. A Top Panel – Tumors were assayed for the presence of Myc-tagged proteins by IHC: Bottom Panel – Tumor lysates were assayed for the presence of MycΔp85α by western blotting. +ve = positive control of a dox induced clone Δ15 cell lysate B The mean (+/− S.E.M) of the tumor growth rate from each group of mice after the tumor reached 300 mm3. C and D Tumors were stained for Ki67 (C) and cleaved PARP (D) by IHC and the percentage of positive cells counted. Data represents the mean +/− S.E.M. * p<0.05, ** p<0.01 compared to all other groups according to two-tailed unpaired t-test.