Table 1.
Radiation chemical yields for free base release from DNA films,with DNA hydration levels of Γ 2.5 to 22.5 waters/nucleotide, X-irradiated at RT.a
| DNA | Form | Γ | G (base) ≡ chemical yield of base in nm/J |
Gtot(fbr) b (nm/J) |
Nc | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cyt | Gua | Thy | Ade | ||||||||||
| pUC18 | film | 2.5 | 41 | (1) | 29.0 | (1) | 34.0 | (1) | 30.0 | (1) | 134.0 | (2.0) | d |
| film | 22.5 | 46 | (2) | 47.0 | (7) | 64.0 | (9) | 55.0 | (5) | 212.0 | (12.6) | d | |
| calf thymus | film | 2.5 | 41 | (2) | 37.0 | (1) | 42.0 | (2) | 42.0 | (2) | 162.0 | (3.6) | e |
| calf thymus | film | 2.5 | 39.6 | (0.9) | 39.0 | (0.7) | 37.4 | (0.9) | 37.3 | (1.1) | 153.3 | (1.8) | 2 |
| d(CGCGCG- CGCG)2 |
film | 2.5 | 38.4 | (0.9) | 78.6 | (2.8) | 117.0 | (3.0) | 3 | ||||
| film | 7.5 | 37.9 | (1.4) | 76.6 | (5.0) | 114.5 | (5.2) | 3 | |||||
| film | 11.3 | 37.0 | (1.7) | 96.0 | (2.3) | 133.0 | (2.9) | 2 | |||||
| film | 15 | 36.5 | (2.1) | 66.0 | (19.5) | 102.5 | (19.6) | 2 | |||||
| film | 22 | 47.6 | (4.6) | 163.0 | (15.6) | 210.6 | (16.3) | 2 | |||||
| crystlf | 7 | 23.2 | (1.0) | 34.7 | (1.9) | 57.9 | (2.1) | 1 | |||||
| d(CGCGAA- TTCGCG)2 |
film | 2.5 | 22.3 | (1.0) | 31.6 | (2.2) | 6.9 | (0.3) | 9.3 | (1.5) | 70.06 | (2.8) | 3 |
| film | 7.5 | 38.0 | (2.0) | 41.8 | (2.2) | 17.0 | (0.6) | 18.6 | (0.7) | 115.4 | (3.1) | 2 | |
| film | 11.3 | 33.2 | (1.4) | 71.0 | (1.5) | 6.2 | (0.2) | 19.7 | (0.6) | 130.1 | (2.1) | 2 | |
| film | 22 | 65.0 | (1.7) | 73.8 | (3.0) | 33.2 | (1.3) | 41.5 | (1.8) | 174.1 | (5.8) | 3 | |
| crystlf | 15 | 30.6 | (1.2) | 76.2 | (5.2) | 11.2 | (1.0) | 56.1 | (2.0) | 213.5 | (4.1) | 1 | |
| Micrococcus luteus |
film | 2.5 | 25.1 | (2.5) | 24.7 | (3.3) | 7.6 | (0.2) | 7.4 | (0.8) | 64.8 | (4.2) | 2 |
| film | 7.5 | 37.8 | (3.8) | 24.1 | (1.0) | 8.1 | (0.2) | 8.9 | (0.3) | 78.9 | (3.9) | 2 | |
| film | 11.3 | 37.5 | (1.4) | 33.8 | (1.4) | 11.6 | (0.4) | 12.1 | (0.4) | 95.0 | (2.1) | 2 | |
| film | 22.5 | 67.4 | (6.4) | 31.0 | (3.4) | 15.9 | (1.1) | 15.4 | (0.5) | 129.8 | (7.3) | 2 | |
| Clostridium perfringens |
film | 2.5 | 24.8 | (2.3) | 12.6 | (0.8) | 12.5 | (0.3) | 19.8 | (1.5) | 69.7 | (2.9) | 2 |
| film | 11.3 | 24.5 | (1.0) | 20.1 | (1.8) | 20.0 | (1.1) | 31.8 | (0.8) | 96.4 | (2.4) | 1 | |
| film | 22.5 | 34.0 | (2.6) | 27.0 | (4.8) | 33.2 | (1.9) | 35.9 | (1.7) | 130.1 | (6.0) | 1 | |