Skip to main content
. Author manuscript; available in PMC: 2011 May 14.
Published in final edited form as: Cell. 2010 May 14;141(4):583–594. doi: 10.1016/j.cell.2010.04.020

Figure 4. In vitro exhaustion after knockdown of JARID1B.

Figure 4

(A) MTS proliferation assays between days 7–10 and days 30–33 after JARID1B knock down (clones 58 and 62, see also Figure S4). Experiments were done in three independent approaches in conventional medium (Tu2%). (B) Exposure of JARID1B knocked down cells (5×104 cells) to hESCM4 resulted in a strong increase of the number of trypan blue-positive cells after 4–6 weeks. (C) Limited (single cell) dilution assays determined the reduction of melanoma sphere formation in JARID1B knockdown cells (right panel). The left panel shows the corresponding cell morphologies. (D) Reduced 3D colony formation after JARID1B knockdown in Tu2% softagar. Depicted are representative results from at least 3 independent experiments.