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. Author manuscript; available in PMC: 2011 May 14.
Published in final edited form as: Cell. 2010 May 14;141(4):583–594. doi: 10.1016/j.cell.2010.04.020

Figure 7. JARID1B affects Jagged 1/Notch 1 signaling.

Figure 7

(A) QPCR of A375-SM melanoma cells transiently transfected with pBIND-JARID1B vs. empty vector control (upper panel) and of JARID1B-knocked down WM3734, WM35, and WM3899 cells vs. sh scrambled control (lower panel). (B) Immunoblotting of JARID1B-transfected A375-SM melanoma and HEK293 control cells with antibodies against JAG1, uncleaved and cleaved Notch 1. Notch cleavage was confirmed by a second antibody. (C) Differential JAG1 expression and Notch cleavage in FACS-isolated J/EGFP-positive and -negative cells. (D) Differential expression of HEY1 and 2 in J/EGFP-positive and -negative cells.