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. Author manuscript; available in PMC: 2010 Jul 6.
Published in final edited form as: Cancer Biol Ther. 2009 Jul 6;8(13):1212–1213. doi: 10.4161/cbt.8.13.8630

Figure 1. Targeted overexpression of the transgene FLAG-hLRAT to the mouse epithelial basal layer cells.

Figure 1

Figure 1

A, The schematic structure of the transgene cassette. B, Southern blot result shows the presence of the transgene in the mouse genomic DNA. C, RT-PCR analysis of the FLAG-hLRAT transgene mRNA in the tongue and skin of K14/FLAG-hLRAT Southern blot transgenic positive (TG+) and negative (TG-) mice from founder #54. D, The expression of the transgene human LRAT protein in the tongue epithelial basal layer cells of K14/FLAG-hLRAT transgenic positive (TG+) and negative (TG-) mice from founder #54 (200 ×). K14/FLAG-hLRAT TG+ and TG- mice were sacrificed, and the tongues were fixed, embedded, sectioned, and stained with anti-human LRAT antibody (see “Materials and Methods”). a, TG positive mouse tongue; b, TG negative mouse tongue; c, negative control, tongue stained only with a secondary antibody; the inset in each picture is 3 × the digital magnification of the small boxed area in the picture. E, the levels of retinyl esters in the skin of K14/FLAG-hLRAT TG positive and negative mice examined by HPLC after a 5 day topical treatment with 40 nmoles (volume 400 μl) of all-trans retinoic acid (ATRA) or retinol per day. Drugs were dissolved in acetone that was the vehicle (see “Materials and Methods”). TG+ #33 and #54 are two TG positive founder lines. The differences among different treatment groups were analyzed by using a one way ANOVA test. Differences with a p value of < 0.05 (marked with an asterisk) in a comparison of retinyl ester levels in retinol treated TG+ mouse skin to that in retinol treated TG- mouse skin were considered to be statistically significant.