Fig. 4.
10,12 CLA-induced inflammatory and ISR gene expression are dependent on [Ca+2]i. A: Cultures of newly differentiated human adipocytes were treated for 12 h with thapsigargin (Tg) in the absence or presence of 100 μM TMB-8 (T). B: Cultures were treated for 12 h with BSA vehicle (V), 50 μM 10,12 CLA alone (10), or 10,12 CLA in the presence of 2 μM BAPTA (10+B), 10 μM KN-62 (10+K), or 100 μM TMB-8 (10+T). A and B: RNA was subsequently isolated and the mRNA levels of IL-8, IL-6, COX-2, ATF-3, CHOP, GADD34, or GAPDH were measured by real-time PCR. Data are normalized to the vehicle controls. Means (± SEM; n = 2) that do not share a common lower case letter differ (P < 0.05). Data are representative of three independent experiments. One-way ANOVA was used to compare data.