Fig. 6.
10,12 CLA activation of the inflammatory PG pathway is not dependent on [Ca2+]i. A: Cultures of newly differentiated human adipocytes were treated with BSA vehicle (V) or 30 μM 10,12 CLA (10) for 6, 12, or 24 h. Cells were harvested and immunoblotted for p-PLA2 and total PLA2. B: Cultures were treated for 12 h with BSA vehicle (V), 30 μM 9,11 CLA (9), or 30 μM 10,12 CLA (10), and immunoblotted as in A. Data are representative of three experiments. C: Cultures were treated for 12 h with BSA vehicle (V), 50 μM 10,12 CLA alone (10), or 10,12 CLA in presence of 2 μM BAPTA (10+B), 10 μM KN-62 (10+K), or 100 μM TMB-8 (10+T), and immunoblotted as in A. D: Cultures were treated for 24 h with BSA vehicle (V), 50 μM 10,12 CLA alone (10), or 10,12 CLA in the presence of 2 μM BAPTA (10+B), 10 μM KN-62 (10+K), or 100 μM TMB-8 (10+T). Conditioned media were subsequently collected and PGF2α levels were measured using a commercially available EIA kit. Means (± SEM; n = 2) not sharing a common lower case letter differ (P < 0.05). Data in all panels are representative of three independent experiments. One-way ANOVA was used to compare data.