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. 2010 Jul;51(7):1859–1870. doi: 10.1194/jlr.M004234

Fig. 1.

Fig. 1.

Activation of SREBP-1c promoter by PMA via −90bp region in the promoter. A: Schematic illustration of mouse SREBP-1c promoter. LXRE complex including two LXRE (LXR responsive element) exists between −250 and −150bp. SRE complex, including NF-Y, E-box, SRE, and GC-box, exists downstream of −90bp. B: Effect of PMA on SREBP-1c promoter activity. HepG2 cells were cotransfected with pBP-1c2600-Luc (containing −2.6kbp region of SREBP-1c promoter). After transfection, PMA (0, 3, 10, 100, 300 nM) or vehicle was added for 20 h. RLU (firefly luciferase activity:renilla luciferase activity ratio) is shown. *P < 0.01 versus vehicle. C: Effect of PMA on several deletion constructs of SREBP-1c Luc. HepG2 cells were cotransfected with pBP-1c2600-Luc, pBP-1c550-Luc, pBP-1c90-Luc, or pGL2basic. After transfection, PMA (100 nM) or vehicle was added for 20 h. Fold activation by PMA is shown with values from vehicle-treated cells set at 1. Relative promoter activity of each construct without PMA is 12.7-fold in pBP-1c2600-Luc, 14.4-fold in pBP-1c550-Luc, and 4.6-fold in pBP-1c90-Luc compared with prompter activity in empty vector. *P < 0.005 versus each promoter construct with no treatment. D: Effect of PMA on −90bp region deleted promoter construct. HepG2 cells were cotransfected with pBP-1c550, pBP-1c90, pBP-1c550 (ΔSRE complex), or pBP-1cTATA-Luc followed by adding PMA (100 nM), T0901317 indicated as T1317 (1 μM), or vehicle for 20 h. RLU (firefly luciferase activity:renilla luciferase activity ratio) is shown. *P < 0.01 versus each promoter construct with no treatment. LUC, luciferase; LXR, liver X receptor; PMA, phorbol-12-myristate-13-acetate; Sp, specificity protein; SRE, sterol regulatory element; SREBP, sterol regulatory element-binding protein; TATA, TATA-like sequence.