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. 2010 Jul;51(7):1859–1870. doi: 10.1194/jlr.M004234

Fig. 6.

Fig. 6.

PKC activation and inhibition altering SREBP-1c expression in rat primary hepatocytes. A: Effect of PKC activation by PMA on SREBP-1c expression in hepatocytes. Rat primary hepatocytes were treated with PMA (0, 0.01, 0.03, 0.1 μM) for 6 h. Total RNA was extracted, and RNA aliquots (10 μg/lane) were subjected to Northern blot analysis using labeled cDNA probes as indicated in figure. B: Effect of PKC selective inhibitor on SREBP-1c expression induced by insulin in rat primary hepatocytes. After overnight serum starvation, rat primary hepatocytes were treated with LY333531 (150 nM) and/or insulin (100 nM) for 24 h under a high glucose (540 mg/dl) medium. Extracted RNA aliquots (10 μg/lane) were subjected to Northern blot analysis using labeled cDNA probes as indicated in figure. NB, Northern blot; PKC, protein kinase C; PMA, phorbol-12-myristate-13-acetate; SOCS3, suppressor of cytokine signaling 3; SREBP, sterol regulatory element-binding protein.