Fig. 7.

Hepatic SREBP-1c expression induced by refeeding abolished PKCbeta selective inhibitor on hepatic SREBP-1 expression. 24-h-fasted mice (control and STZ-treated mice) were followed by 12-h refeeding. Food was prepared as normal diet (Cont) and LY333531-contained normal diet (LY). mRNA level of hepatic SREBP-1 in Cont or LY administrated control and STZ mice. Extracted liver total RNA was pooled and RNA aliquots (10 μg/lane) were subjected to Northern blot analysis using labeled cDNA probes as indicated in figure (n = 4–6). NB, Northern blot; PEPCK, phosphoenolpyruvate carboxykinase; PKC, protein kinase C; Sp, specificity protein; SREBP, sterol regulatory element-binding protein; STZ, streptozotocin.