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Solubilization of phospholipid vesicles by MPO-oxidized apoA-I. Control or oxidized apoA-I samples were added to a solution of DMPC vesicles to give a final DMPC/apoA-I ratio of 2:1 (w/w) at a final protein concentration of 0.17 mg/ml. The experiment was performed at 24.5°C with monitoring of absorbance at 325 nm. Oxidation reactions were carried out at a 25:1 [mol:mol] ratio of oxidant to apoA-I. Results represent two independent experiments.
