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. 2010 Jul;51(7):1886–1896. doi: 10.1194/jlr.M004978

Fig. 2.

Fig. 2.

Expression of ACSL3, 5, and 6 are increased with synthetic LXR agonist, but only ACSL3 is predominantly expressed in BeWo cells. A: BeWo cells were incubated with vehicle (white bars), 9-cis RA (1 μM, gray bars), GW3965 (1 μM, striped bars), or both agonists together (black bars) for 48 h. Total RNA was analyzed for gene expression of the five ACSL isoforms by qRT-PCR normalized to TBP. The results are presented as means of three independent experiments performed in triplicate (n = 3) ± SEM relative to control. *P < 0.05 and **P < 0.01 B: Basal expression of ACSL, FATP, and ACSBG family members in BeWo cells (white bars) and placenta (black bars) normalized to B2M. The results are represented as 2(-delta Ct) for the means of three independent BeWo cell experiments performed in triplicate (n = 3) and for the means of six independent placenta isolations performed in triplicate (n = 6) ± SEM. Ct values are indicated for placental tissue. ACSBG, acyl-CoA synthetase bubblegum; ACSL, long-chain acyl-CoA synthetase; B2M, beta-2-microglobulin; BeWo, human placental choriocarcinoma; FATP, fatty acid transport protein; LXR, liver X receptor; qRT-PCR, quantitative reverse transcription-PCR; SREBP-1, sterol-regulatory element binding protein-1; TBP, TATA box binding protein.