Fig. 3.
Intestines from Pomc-Cre Leprfl/fl mice secrete fewer lipids with apoB-containing lipoproteins. Pomc-Cre and WT mice (n = 3) fasted overnight were injected intraperitoneally with poloxamer 407 (45 mg/mouse). After 1 h, mice were fed 1 µCi [3H]triolein in 15 µL of olive oil. Plasma was collected after 2 h and used to measure radioactivity (Panels A–C) as well as triglyceride (Panels D–F) mass in total plasma, non-HDL, and HDL lipoproteins. In another experiment, overnight fasted Pomc-Cre and WT mice were injected with poloxamer 407 and plasma was collected after 3 h to determine hepatic lipoprotein production. Triglyceride was measured in total plasma (Panel G), non-HDL (Panel H), and HDL (Panel I) lipoproteins. Each measurement was done in triplicate with three mice per group. Values are mean ± SD, *P < 0.05, **P < 0.01 compared with WT. LEPR, leptin receptor; MTP, microsomal triglyceride transfer protein; POMC, proopiomelanocortin; WT, wild-type.