Fig. 4.

Partial intestinal deficiency of MTP decreases lipid absorption. Intestinal (Panels A and B) and hepatic (Panels C and D) MTP activity (Panels A and C) and mRNA (Panels B and D) were determined in overnight fasted Mttp^fl/fl and villin-CRE mttp^fl/+ mice (n = 3). In another independent experiment, Mttp^fl/fl and Villin-CRE mttp^fl/+ mice (n = 3) fasted overnight were injected intraperitoneally with poloxamer 407 (45 mg/mouse). After 1 h, mice were fed 1 µCi [³H]triolein in 15 µL of olive oil. Plasma was collected after 2 h and used to measure radioactivity (Panels E and F) in total plasma and non-HDL lipoproteins. Each measurement was done in triplicate with three mice per group. Values are mean ± SD, *P < 0.05, **P < 0.01 compared with Villin-CRE mttp^+/+. MTP, microsomal triglyceride transfer protein.