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. 2010 Jul;51(7):1929–1942. doi: 10.1194/jlr.M005744

Fig. 7.

Fig. 7.

Enterocytes express LEPR, POMC, AGRP, and MC4R. Semiquantitative RT-PCR was used to determine the expression of LEPR-B, POMC, AGRP, and MC4R in the hypothalamus, intestine, enterocytes, and smooth muscle cells (Panel A). Immunohistochemistry to visualize expression of POMC (Panel B) and LEPR (Panel C) in Lepr-CRE Rosa26-floxed GFP mice was performed as described in “Materials and Methods.” Expression of LEPR, POMC, AGRP, and MC4R was also determined by semiquantitative RT-PCR in the enterocytes isolated from WT and Pomc-Cre Leprfl/fl mice (Panel D). Quantitative RT-PCR was used to measure changes in the expression of these genes in enterocytes (Panel E). Expression of LEPR, POMC, AGRP, and MC4R was also determined by semiquantitative RT-PCR in the liver isolated from WT mouse (Panel F). AGRP, agouti gene related peptide; LEPR-B, leptin receptor B; MC4R, melanocortin 4 receptor; POMC, proopiomelanocortin; WT, wild-type.