Figure 1.
Typical purification procedure of GIMAP2 constructs using GIMAP21–260 as an example. 4–12% SDS–PAGE of various samples taken during purification. −/+ IPTG, whole-cell bacteria lysates before and after induction; M, molecular-weight markers, bands correspond to 200, 116, 97, 66, 55, 36.5, 31, 21.5, 14 and 6 kDa (from top to bottom); SN, supernatant of bacterial lysates after ultracentrifugation; NB, nonbound material after GSH-Sepharose column; W, wash fraction of the same column; E1, eluate after protease cleavage containing the GIMAP construct; E2, GSH elution showing GST and the GST-tagged protease.