Figure 3. κB-Ras2 is up-regulated by estradiol.
(A) Total RNA was extracted from macrophages treated with LPS at indicated times. κB-Ras2 mRNA was measured by TaqMan real-time PCR. Values are relative to unstimulated controls. (B) Immunoblot analysis of κB-Ras2 protein levels in response to LPS. (C–D) mRNA and protein levels, respectively, of κB-Ras2. Macrophages were treated with estradiol prior to LPS stimulation for 8 hours (C) or 12–24 hours (D). Data shown are representative of 4 experiments. *p< 0.05 vs. unstimulated control