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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Immunol. 2010 Mar 29;184(9):5029–5037. doi: 10.4049/jimmunol.0903463

Figure 4. Let-7a and miR-125b are regulated by LPS.

Figure 4

(A) Total RNA was extracted from macrophages using Qiagen miRNeasy kits. Let-7a and miR-125b expression were measured using Taqman miRNA assay system. Data are normalized to U6 expression levels. (B) let-7a and (C) miR-125b expression levels in response to LPS stimulation. Data were normalized to U6 and are presented as relative to unstimulated levels. (D–E) Macrophages from a representative donor were treated with NF-κB activation inhibitor (10 μM) for 1 hour prior to LPS stimulation for (D) 12 hours or (E) 3 hours. *p<0.05 vs. unstimulated controls.

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